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  • Cappable-seq Service now available !

    In close cooperation with New England Biolabs, Inc. (NEB), VERTIS is now able to make the new Cappable-seq method available to its customers. The method was developed by Laurence Ettwiller and Ira Schildkraut from NEB (Ettwiller, L. et al. 2016. BMC Genomics. 17, 1 (2016), 199).

    It represents the current most sensitive and robust method for the precise identification of bacterial transcription start sites (TSSs).

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WTSS Small RNA
dRNA-seq Cappable-seq tagRNA-seq

Whole Transcriptome Shotgun Sequencing

Already since 10 years, VERTIS prepares random-primed cDNA libraries in a strand specific manner. We have developed a method which allows the ligation of 5' and 3' sequencing adapters specifically to the 5' and 3' ends of singles-stranded first strand cDNA. The method is outlined in the schema below.

 

 

 

Advantage of method

  • High efficiency, allowing the processing of low amounts of starting material
  • No loss of information from 5'-ends of transcripts
  • Good coverage also of small RNA fraction (> 80 nt)
  • High strand-specifity
 
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