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  • Cappable-seq Service now available !

    In close cooperation with New England Biolabs, Inc. (NEB), VERTIS is now able to make the new Cappable-seq method available to its customers. The method was developed by Laurence Ettwiller and Ira Schildkraut from NEB (Ettwiller, L. et al. 2016. BMC Genomics. 17, 1 (2016), 199).

    It represents the current most sensitive and robust method for the precise identification of bacterial transcription start sites (TSSs).

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Special Applications

VERTIS relies on an innovative in-house developed cDNA platform, which is subjected to a continuous process of further improvement and optimization. This way we can offer highest flexibility to our clients in the realization of their research projects.

Please find here some more detailed information about our different methods for cDNA synthesis and automated library preparation:


  • Dual RNA-seq
Quantifying mixed transcriptomes, e.g. RNA-seq analysis of host-pathogen interactions.

References:

Westermann, A.J. et al. "Dual RNA-seq unveils noncoding RNA functions in host-pathogen interactions." Nature 529, 7587 (Jan. 2016), 496–501.

  • Metatranscriptomics

Analysis of the transcriptome of environmental samples and complete communities.

References:

Bremges, Andreas, et al. "Deeply sequenced metagenome and metatranscriptome of a biogas-producing microbial community from an agricultural production-scale biogas plant." GigaScience 4.1 (2015): 1-6.

Dyksma, Stefan, et al. "Ubiquitous Gammaproteobacteria dominate dark carbon fixation in coastal sediments." The ISME journal (2016).

Sheibani-Tezerji, Raheleh, et al. "Transcriptome Profiling of the Endophyte Burkholderia phytofirmans PsJN Indicates Sensing of the Plant Environment and Drought Stress." mBio 6.5 (2015): e00621-15.

  • Quantitative RNA-seg

Elimination of amplification noise by counting absolute numbers of molecules using unique molecular identifiers (UMI) as described by Islam, S. et al. 2014. Nature Methods. 11, 2 (2014), 163–166.

 

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