VERTIS relies on an innovative in-house developed cDNA platform, which is subjected to a continuous process of further improvement
and optimization. This way we can offer highest flexibility to our clients in the realization of their research projects.
Please find here some more detailed information about our different methods for cDNA synthesis and automated library preparation:
Quantifying mixed transcriptomes, e.g. RNA-seq analysis of host-pathogen interactions.
Westermann, A.J. et al. "Dual RNA-seq unveils noncoding RNA functions in host-pathogen interactions." Nature 529, 7587 (Jan. 2016), 496–501.
Analysis of the transcriptome of environmental samples and complete communities.
Bremges, Andreas, et al. "Deeply sequenced metagenome and metatranscriptome of a biogas-producing microbial community from an agricultural production-scale biogas plant." GigaScience 4.1 (2015): 1-6.
Dyksma, Stefan, et al. "Ubiquitous Gammaproteobacteria dominate dark carbon fixation in coastal sediments." The ISME journal (2016).
Sheibani-Tezerji, Raheleh, et al. "Transcriptome Profiling of the Endophyte Burkholderia phytofirmans PsJN Indicates Sensing of the Plant Environment and Drought Stress." mBio 6.5 (2015): e00621-15.
Elimination of amplification noise by counting absolute numbers of
molecules using unique molecular identifiers (UMI) as described by
Islam, S. et al. 2014. Nature Methods. 11, 2 (2014), 163–166.
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