Special emphasis of VERTIS lies in the analysis of bacterial
transcriptomes. Bacteria are in the central focus of important social
challenge such as infectious diseases and they are of enormous
economic interest as for white biotechnology or food industry.
On the other hand, the preparation of high-quality cDNA from bacteria is
highly demanding, because the bacterial mRNA is very unstable and due
to the absence of 3' poly(A) tails, it's enrichment is much more challenging.
VERTIS has developed efficient techniques
which allow a very accurate and deep analysis of bacterial whole transcriptomes, small regulatory RNAs (sRNome) and transcription start sites (TSS).
Please find here some more detailed information about our different
methods for cDNA synthesis and automated library preparation:
Identification of the Structure of Bacterial Transcriptomes
- Enrichment of primary transcripts:
- dRNA-seq (differential RNA-seq)
- Cappable-seq (direkt targeting of 5' tri-phosphate ends of transcripts)
- Identification of bacterial transcription start sites (TSSs) and processing sites (PSSs) in parallel:
- tagRNA-seq (with focus on identification of TSS and PSS)
- tagRNA-seqWT (whole transcriptome sequencing including information on TSS and PSS)
For more Information