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  • Cappable-seq Service now available !

    In close cooperation with New England Biolabs, Inc. (NEB), VERTIS is now able to make the new Cappable-seq method available to its customers. The method was developed by Laurence Ettwiller and Ira Schildkraut from NEB (Ettwiller, L. et al. 2016. BMC Genomics. 17, 1 (2016), 199).

    It represents the current most sensitive and robust method for the precise identification of bacterial transcription start sites (TSSs).

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Preparation of RNA for cDNA synthesis

RNA isolation

Make use of our over 16 years of experience in the isolation of high quality RNA from your valuable and challenging starting materials.

We have establishes a portfolio of well tested protocols for:

  • Isolation of total RNA from a wide variety of starting materials (animals, plants, algae, fungi, protists, bacteria)

  • Isolation of small non-coding RNA, especially miRNA

  • RNA Isolation from mixed samples (e.g. infected tissues)

  • RNA Isolation from environmental samples (e.g. stuhl, sea water, glacial ice, etc.)

  • Isolation of total RNA from FFPE samples

Each RNA preparation will be quality checked on a Shimadzu MultiNA microchip electrophoresis system and by UV/Vis spectroscopy.

 

Enrichment strategies for different RNA species

Eukaryotic RNA

RNA species

Enrichment strategy

 Poly(A)+

Oligo(dT) chromatography

 Full-length mRNA

CAP selection (enzymatic)

 Small non-coding RNA (sncRNA)

Selective elution from silica gel (< 200 nt)

 MicroRNA

Size fractionation (15 -30 nt) with an automated fractionation system

 rRNA depleted total RNA

Removal or rRNA molecules with subtractive hybridization (Ribo-Zero)

 

Prokaryotic RNA

RNA species

Enrichment strategy

 5'PPP primary transcripts

Enzymatic selection of 5'PPP ends

 Small non-coding RNA (sncRNA)

Selective elution from silica gel (< 200 nt)

 rRNA depleted total RNA

Removal or rRNA molecules with subtractive hybridization (Ribo-Zero)

   
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