Dual RNA-seq unveils noncoding RNA functions in host–pathogen interactions
A. J. Westermann, K. U. Förstner, F. Amman.,L.Barquist, Y.Chao, L.N. Schulte,L.Müller, R. Reinhardt,P. F. Stadler & J. Vogel. Nature (2016) doi:10.1038/nature16547
In close cooperation with New England Biolabs, Inc. (NEB), VERTIS is now able to make the new Cappable-seq method available to its customers. The method was developed by Laurence Ettwiller and Ira Schildkraut from NEB (Ettwiller, L. et al. 2016. BMC Genomics. 17, 1 (2016), 199).
It represents the current most sensitive and robust method for the precise identification of bacterial transcription start sites (TSSs).
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currently the most sensitive and robust method for the precise identification of bacterial transcription start sites (TSSs) | |
A modified RNA-seq approach enabling to discriminate primary from processed 5' RNA ends | |
Elimination of amplification noise |
Analysis of eukaryotic and prokaryotic transcriptomes
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Strand specific sequencing from poly(A)+ or rRNA depleted RNA |
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Full-lenght cDNA for sequencing with the PacBio system |
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For equalization of gene representation in NGS libraries |
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Digital Gene Expression analysis |
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Identification of miRNA targets |
Professional miRNA sequencing services | |
Identification of bacterial regulatory sRNAs (sRNome) |
Identification of the structure of bacterial transcriptomes
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Differential RNA-seq for annotation of transcriptional start sites |
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Most sensitive and robust method for the identification of bacterial TSS |
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Method to discriminate primary from processed 5' RNA ends |
Special applications
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Quantifying mixed transcriptomes |
Analysis of environmental samples and complete communities | |
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Elimination of amplification noise |
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Isolation of high quality RNA from all kinds of strating materials |