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  • Cappable-seq Service now available !

    In close cooperation with New England Biolabs, Inc. (NEB), VERTIS is now able to make the new Cappable-seq method available to its customers. The method was developed by Laurence Ettwiller and Ira Schildkraut from NEB (Ettwiller, L. et al. 2016. BMC Genomics. 17, 1 (2016), 199).

    It represents the current most sensitive and robust method for the precise identification of bacterial transcription start sites (TSSs).

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WTSS FL-cDNA Normalization MicroRNA PARE DGE

Whole Transcriptome Shotgun Sequencing

Already since 10 years, VERTIS prepares random-primed cDNA libraries in a strand specific manner. We have developed a method which allows the ligation of 5' and 3' sequencing adapters specifically to the 5' and 3' ends of singles-stranded first strand cDNA. The method is outlined in the schema below.

 

 

 

Advantage of method

  • High efficiency, allowing the processing of low amounts of starting material
  • No loss of information from 5'-ends of transcripts
  • High strand-specifity

References

Cavalier-Smith, T. et al. 2015. Multigene phylogeny resolves deep branching of Amoebozoa. Molecular Phylogenetics and Evolution. 83, (2015), 293–304.

Behrend, A. et al. 2015. “The usual suspects″- analysis of transcriptome sequences reveals deviating B gene activity in C. vulgaris bud bloomers. BMC Plant Biology. 15, 1 (2015), 8.

Fritz, M. et al. 2015. Novel insights into RNP granules by employing the trypanosome‘s microtubule skeleton as a molecular sieve. Nucleic Acids Research. 43, 16 (2015), 8013–8032

Wibberg, D. et al. 2014. Transcriptome analysis of the phytopathogenic fungus Rhizoctonia solani AG1-IB 7/3/14 applying high-throughput sequencing of expressed sequence tags (ESTs). Fungal Biology. 118, 9-10 (2014), 800–813.

Christensen, K. and Anistoroaei, R. 2014. An American mink (Neovison vison) transcriptome. Animal Genetics. 45, 2 (2014), 301–303.

Posnien, N. et al. 2014. A Comprehensive Reference Transcriptome Resource for the Common House Spider Parasteatoda tepidariorum. PLoS ONE. 9, 8 (2014), e104885.

Taylor, A. et al. 2010. Conservation of Arabidopsis thaliana Photoperiodic Flowering Time Genes in Onion (Allium cepa L.). Plant and Cell Physiology. 51, 10 (2010), 1638–1647.

 
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