Whole Transcriptome Shotgun Sequencing
Already since 10 years, VERTIS prepares random-primed cDNA libraries in a strand specific manner. We have developed a method which allows the ligation of 5' and 3' sequencing adapters specifically to the 5' and 3' ends of singles-stranded first strand cDNA. The method is outlined in the schema below.
Advantage of method
- High efficiency, allowing the processing of low amounts of starting material
- No loss of information from 5'-ends of transcripts
- High strand-specifity
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