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  • Cappable-seq Service now available !

    In close cooperation with New England Biolabs, Inc. (NEB), VERTIS is now able to make the new Cappable-seq method available to its customers. The method was developed by Laurence Ettwiller and Ira Schildkraut from NEB (Ettwiller, L. et al. 2016. BMC Genomics. 17, 1 (2016), 199).

    It represents the current most sensitive and robust method for the precise identification of bacterial transcription start sites (TSSs).

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WTSS FL-cDNA Normalization MicroRNA PARE DGE

Sequencing-based RNA expression profiling

NGS has the potential to replace microarrays as the method for transcriptome profiling. The major advantages of DGE over microchip-based approaches are:

  • increase of the dynamic range of analyses (5 orders of magnitude)
  • ability to detect novel exons  (truly genome wide expression profiling)
  • no demand for a priori sequence information (gene expression in any species)
  • generates expression data plus sequence information at the same time
    (parallel detection of SNPs which can be used for genotyping experiments)
  • no false positives

 

We offer sequencing of 3'-fragment cDNA which starts 100 - 200 bp upstream of the polyadenylation sites of the transcripts.

Because each transcript is represented by only a single tag, 3'-fragment libraries are substantially less complex than standard RNA-Seq libraries, allowing useful results with fewer reads per sample (about 10 million reads per transcriptome), thus making 3'DGA much more cost efficient.

 
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